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STANDARD OPERATING PROCEDURES Safe Autoclave Operations

     The purpose of this document is to provide standard operating procedures for the
safe use of autoclaves. Autoclaving is a process used to destroy microorganisms and
decontaminate biohazardous waste and microbiological equipment used at
Biosafety Level 1 / 2.
Autoclave Make/Model:
EX:  •Consolidated Stills & Sterilizers, Model # SSR3APB
Serial #42090
Person Responsible and Phone #: ______________
Located in Building/Room: Building ____, room ____


HAZARDS       Autoclaves use high pressure (15 psi) and high temperature(121 C) or 250 F)  steam for sterilization.
Potential safety risks for the operators include:
          · Heat burns from hot materials and autoclave chamber walls and door.
          · Steam burns from residual steam coming out from autoclave and materials
          on completion of cycle.
          · Hot fluid scalds from boiling liquids and spillage in autoclave.
          · Hand and arm injuries when c…
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Microbiology Laboratory Safety
1). Objectives Students successfully completing this laboratory exercise should be able to: 1. Locate and properly use basic emergency equipment such as eye wash stations, first aid kits, fire extinguishers, a telephone, etc. 2. Wear proper attire for the microbiology lab at all times (lab coat that comes down to knee’s, goggles, gloves). 3. Wash hands prior to and following laboratories and at any time contamination is suspected. 4. Never eat or drink in the laboratory. EVER. 5. Never apply cosmetics, handle contact lenses, place objects (fingers, pencils, etc.) in the mouth, or touch the face. 6. Report all injuries immediately to the instructor. 7. Report all spills or broken glassware to the instructor and receive instructions for clean-up. 8. Disinfect the lab benches prior to and after laboratory exercises. 9. Keep lab benches clear of extraneous materials. 10. Follow appropriate steps in the event of an accident. 11. Lab coats stay in the lab!! If you want to t…