STANDARD OPERATING PROCEDURES Safe Autoclave Operations The purpose of this document is to provide standard operating procedures for the safe use of autoclaves. Autoclaving is a process used to destroy microorganisms and decontaminate biohazardous waste and microbiological equipment used at Biosafety Level 1 / 2. Autoclave Make/Model: EX: •Consolidated Stills & Sterilizers, Model # SSR3APB Serial #42090 Person Responsible and Phone #: ______________ Located in Building/Room: Building ____, room ____ HAZARDS Autoclaves use high pressure (15 psi) and high temperature(121 C) or 250 F) steam for sterilization. Potential safety risks for the operators include: · Heat burns from hot materials and autoclave chamber walls and door. · Steam burns from residual steam coming out from autoclave and materials on completion of cycle. · Hot fluid scalds from boiling liquids and spillage in autoclave. ·