Growth at Various Temperatures

All four plates were inoculated with the same 4 organisms and incubated at the various temperatures (4, 25, 40 and 60 degree's C for 24 hours) on TSA agar:

Upper left Quadrent:  Staphylococcus aureus                   

Upper Right Quadrent:  Bacillus  stearothermophilus, make sure to parafilm these plate's or they will dry out.

Lower Left Quadrent:  Pseudomonas  fluorescens, this bacteria is a slow grower at refrigerated temp's and can take a week plus to get good results.

Lower Right Quadrent:  E. coli


4 Degree's C



Psychrophiles (cold loving):  Bacterial species that will grow within a temperature range of -5oC to 20oC.  The distinguishing characteristics of all psychrophiles is they will grow between 0o and 5oC.  Capable of living and growing at cold temperatures they are responsible for food spoilage in refrigerators.

25 Degree's C

Psychrotrophs:  Bacterial species that will grow within a temperature range of 0oC to 35oC.  Optimal temperature is 25oC.

40 Degree's C
Mesophile:  Bacterial species that will grow within a temperature range of 20oC to 45oC.
Distinguishing characteristics of all mesophiles are their ability to grow at human body temperature (37oC).

60 Degree's C

Thermophiles: Bacterial species that will grow within 40oC to 122oC with optimal growth from 65oC to 70oC.

Extreme Thermophiles:  Bacterial species that will grow within 65oC to 110oC with optimal temperature around 100oC.  These bacteria are normally found in geothermal area's such as YellowStone.
 One of the many beauty pools seen in the Yellowstone caldera, Wyoming.  Bacterial mats, golden brown in color, seen on the outer edges of two different chromatic Beauty Pool's in Upper Geyser Basin. The blue water in the center of each pool is the hottest water at ~199 °F cooling to ambient the farther from the center. Cyanobacteria grow around 163 °F or lower.


(A)

(B)

Yellowstone caldera, Wyoming. (A) Golden brown thermophilic cyanobacterial mat, morphology was mucoid/slimy, seen in the run off water pool surrounding one of the geyser cones (B) in Upper Geyser Basin. The geyser water run off can be seen in (B) as brown streaks of cyanobacterial growth down the side of the geyser cone.

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