Growth at Various Temperatures

All four plates were inoculated with the same 4 organisms and incubated at the various temperatures (4, 25, 40 and 60 degree's C for 24 hours) on TSA agar:

Upper left Quadrent:  Staphylococcus aureus                   

Upper Right Quadrent:  Bacillus  stearothermophilus, make sure to parafilm these plate's or they will dry out.

Lower Left Quadrent:  Pseudomonas  fluorescens, this bacteria is a slow grower at refrigerated temp's and can take a week plus to get good results.

Lower Right Quadrent:  E. coli


4 Degree's C



Psychrophiles (cold loving):  Bacterial species that will grow within a temperature range of -5oC to 20oC.  The distinguishing characteristics of all psychrophiles is they will grow between 0o and 5oC.  Capable of living and growing at cold temperatures they are responsible for food spoilage in refrigerators.

25 Degree's C

Psychrotrophs:  Bacterial species that will grow within a temperature range of 0oC to 35oC.  Optimal temperature is 25oC.

40 Degree's C
Mesophile:  Bacterial species that will grow within a temperature range of 20oC to 45oC.
Distinguishing characteristics of all mesophiles are their ability to grow at human body temperature (37oC).

60 Degree's C

Thermophiles: Bacterial species that will grow within 40oC to 122oC with optimal growth from 65oC to 70oC.

Extreme Thermophiles:  Bacterial species that will grow within 65oC to 110oC with optimal temperature around 100oC.  These bacteria are normally found in geothermal area's such as YellowStone.
 One of the many beauty pools seen in the Yellowstone caldera, Wyoming.  Bacterial mats, golden brown in color, seen on the outer edges of two different chromatic Beauty Pool's in Upper Geyser Basin. The blue water in the center of each pool is the hottest water at ~199 °F cooling to ambient the farther from the center. Cyanobacteria grow around 163 °F or lower.


(A)

(B)

Yellowstone caldera, Wyoming. (A) Golden brown thermophilic cyanobacterial mat, morphology was mucoid/slimy, seen in the run off water pool surrounding one of the geyser cones (B) in Upper Geyser Basin. The geyser water run off can be seen in (B) as brown streaks of cyanobacterial growth down the side of the geyser cone.

All images Copyr





Tee Shirt available at Cabrillo College Book Store(831479-6209
or
Niles Biological:  http://www.nilesbio.com/cat499.html 

All images copyright T. Sturm















 

Comments

Post a Comment

Popular posts from this blog

Image
  Basic Bacterial Morphology  Bacteria and fungus (mold and yeast) grow in a variety of shapes, sizes and colors.  Although it is difficult to make a definite identification of cultures growing in broth or solid media many have distinguishing characteristics that give us clues to identification.  Many Microbiologist can make a tentative identifications by growth characteristics but definitive id requires further testing (ie Gram stain, chemical testing, etc). The palm of a hand print print done on TSA agar.  There is a mix of different textured and colored colonies on the plate some bacterial some yeast.  By looking at color, texture, growth times and various growth temperatures you can get an idea as to what is on the plate.  Ex:  the orange colonies are yeast and tend to grow better at room temperature or refrigerated temperatures where the large colony on the left ( Bacillus spp) and the white colonies ( Staphylococcus ) grows well at body temperature.  Further testing need
Read more
Image
Oxygen Requirements:  Fluid  Thioglycolate Media A                             B                                C                                      D                                E    Organisms grown in Fluid Thioglycollate Media, a reducing media that uses sodium thioglycolate to chemically combine with dissolved oxygen to deplete the oxygen in the media. The top portion of the tube contains oxygen while the bottom of the tube is lacking molecular oxygen.  All organism except for  Neisseria sicca  were grown for 48 hours at 37 o  C. Neisseria sicca  was grown for 72 hours at 37 o  C for best results.  Note: Both  N. sicca  and  C. butrycium  were grow in supplemented Thioglycollate Media.  A calcium chip can be seen in the bottom of the tube for  N. sicca which is used as a buffer.  This media is aslo supplemented with vitamin K.   Note:  I buy prepared thioglycolate media and many times the media is "jostled" in shipping.  I will autoclave the tubes to d
Read more
Image
Nitrate Reduction/Nitrate Reductase Test Some bacterial species are able to reduce nitrate (NO3)  to nitrite (NO2)  using the enzyme nitrate reductase in an anaerobic process by using molecules other then oxygen, nitrate, as a terminal electron acceptor.  To determine if a bacteria is able to reduce nitrate they are grown in a nitrate broth and a series of chemical are added to detect nitrate reduction. (A)                                                 (B)                                                     (C)      Nitrate reduction test for the reduction of nitrate in nitrate media, to determine if the bacteria contains nitrate and/or nitrite reductase. Organism were incubated for 48 hr's at 37 degree's in nitrate media then reagents added.  The addition of these reagents test for the presence of nitrites and the media will turn red if nitrites are present. Nitrate broths after the addition of 10 drops reagents A (sulfanilic acid in acetic acid) and B (nn-di
Read more